Development of "green" methods for determining pesticides in environmental objects based on vacuum solid-phase microextraction

Relevance of the project:

The development of "green" methods for determining organic pollutants in environmental objects is a broad area of scientific research in analytical chemistry. The relevance of such research is justified by the fact that standard methods for determining organic substances in complex matrices are based on complex and labor-intensive sample preparation methods, requiring the use of large volumes of toxic organic solvents. The development of "green" analytical methods is particularly important for currently used pesticides, which are subject to constant regulatory control and monitoring.
Headspace solid-phase microextraction (HS-SPME) is a promising solvent-free sample preparation method that combines extraction, concentration, and purification in one operation. The main problems of quantitative determination of pesticides by HS-SPME are low analysis accuracy caused by the matrix effect and low extraction efficiency of analytes with high boiling points and low Henry's constants.
In the proposed project, the method of combined vacuum HS-SPME (Vac-HS-SPME) and cold fiber SPME will be used to increase the sensitivity and reproducibility of pesticide determination in environmental objects. To increase the accuracy of analyte determination and control the matrix effect, calibration by the method of multiple Vac-HS-SPME will be used. The results of studying the kinetics and mechanisms of Vac-HS-SPME and cold fiber SPME for various classes of pesticides can form the basis for the development of economically efficient and accurate "green" methods for determining other analytes in various matrices. The implementation of the developed methods in laboratories engaged in determining residual amounts of pesticides in various objects within the framework of state regulation will reduce the cost, time, and labor of analysis.